Sally:
The simple answer to the question of whether or not biotin blocking is
required is ‘no’. As you stated in your inquiry, blocking need only be
performed when excessive nonspecific staining, attributable to endogenous
biotin,
is observed.
For what its worth, I think that some folks
We use biotin blocking only with certain tissues, i.e. liver, kidney,
GI, or diseases, i.e. oncocytomas.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
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