...@gmail.com]
Verzonden: woensdag 6 juni 2012 1:31
Aan: histonet@lists.utsouthwestern.edu
Onderwerp: Re: [Histonet] Fluorescence-filters
Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for
brightfield).
Emily
You see a peanut, day's off to a good start
Of Emily Sours
Sent: Tuesday, June 05, 2012 7:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fluorescence-filters
Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for
brightfield).
Emily
You see a peanut, day's off to a good
Hi Gudrun:
What you wrote is news for me.
Dichroic filters with specific lambda transmission of good quality can be used
for years.
The filters I used were Ernst Leitz, from the early 1950's and the transmission
intensity remained strong.
We checked the life duration of the mercury lamp, but not
Filters are usually very stable. What is burning out are fiber optic cables
that are present in some illumination systems such as X-Cite in Zeiss
microscopes. These optical fiber cables should be replaced after 2000 hours.
Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs,
Hi Gudrun,
i read about 15 ALK cases a week. If you are seeing a lot of collagen
fibers around the tumor cells, I'd try increasing the digestion time of
your pepsin (especially if they were fixed for longer than usual). Before
altering the pretreatment though, you would want to make sure that
Wow, that was detailed and interesting!!
I didn't know microscopes could even use LED, does that require a different
setup, or just a different bulb?
Emily
You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty
Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for
brightfield).
Emily
You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir.
--Howard Moon, in Charlie, The Mighty Boosh
