RE: [Histonet] Formic Acid Recipe

2011-11-06 Thread Patsy Ruegg
Jesus, The formic acid (5%) will decalcify the hydroxyapatite which can be very hard and crunchy to cut but since you ground the section I guess you don't care about that. I would be concerned though that the formic acid might also cause you to lose staining of some cell components that depend on

RE: [Histonet] Formic Acid Recipe

2011-11-04 Thread Jack Ratliff
Hello Jesus! Try Sanderson's Rapid Bone Stain from Dorn and Hart Microedge (www.dornandhart.com). This is a metachromatic stain and should help you to penetrate the resin if heated at 60C and stained for 5-10 minutes depending upon section thickness. Rinse the slide in dH2O @ 60C and blot dry