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-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
Sent: Thursday, December 11, 2014 11:19 AM
To: 'Joanna'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Gomori Trichrome
Joanna, If it looks like it is under the section, then I would suspect t
Joanna, If it looks like it is under the section, then I would suspect trapped
reagent in air bubbles, folds, etc. If it is on top of the section then maybe
more rinsing is needed.
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical C
Hi,
you can try, if you do not already, to wash briefly
in a solution of 0.2 ml. of glacial acetic acid in
100 ml. of distilled water, before the final dehydration.
That makes the colors more delicate and transparent.
The Gomori's trichromic results are similar to those obtained
with the Masson'