Re: [Histonet] Helicobacter helmani -- help?

All - thank you for the education! We're waiting for a definite response from the manufacturer and will offer an Alcian yellow while we wait... Happy holidays!! Sent from my iPhone > On Dec 4, 2013, at 6:01 PM, Milanka Ilic wrote: > > Hi Cheryl, > > The polyclonal Helicobacter pylori (

RE: [Histonet] Helicobacter helmani -- help?

We had same problem at my old job. I don't think they have come up with a separate IHc so our doctor would order an alcian yellow with ihc on children under age of 12 I think but we gave this up cause it's so rare. If you look up on Google under h. Heilmannii, I believe its spelled, it has a

RE: [Histonet] Helicobacter helmani -- help?

The polyclonal antibody from Dako labels H. helmennia in addition to H. pylori. Several of the commercially-available mAbs to H. pylori that I have evaluated do not label H. helmennia. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Pro

Re: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining

Gudrun: Were the cases to be known as positive, or were you trying to determine just that? If they were known as (+) then the bacteria should be seen outside the cells. If you were trying to determine if they were (+) and you do not see the organisms it is just that those cases were (-), especial

Re: [Histonet] Helicobacter pylori

Hello, We use what is pretty much a diff quik stain, the stain is called QW and it is from poly scientific. The procedure is really easy. Deparaffinize, alcohols, methanol, water and then we do 15 slow dips in the QW2, 15 slow dips in the QW3, wash in water, 2 quick dips in alcohol and then dry

RE: [Histonet] Helicobacter pylori

We basically do a dif quik stain. I buy a kit called "Three Step Stain" from Cardinal. The total staining process takes about 1 minute. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M

RE: [Histonet] Helicobacter pylori

We use an Acridine Orange stain. You will need a fluorescent scope for it, though. It's a very simple procedure. Depariffinize slides, air dry, apply AO solution, let stand for 7 minutes, rinse, dry, coverslip from xylene. -Original Message- From: histonet-boun...@lists.utsouthwestern.

Re: [Histonet] Helicobacter Pylori antibody

We use Cell Marque's Polyclonal H. Pylori  (CMC435) at a 1:100 dilution on the Ventana BenchMark for 32 minutes, Protease 1 for 12 minutes.   ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson,  AZ ~Don't let your life end before it begins~ OU Rocks __

RE: [Histonet] Helicobacter Pylori antibody

We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20 minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare) 10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes hematoxylin(Dako).

RE: [Histonet] Helicobacter Pylori antibody

Hi Chakib, We use Ventana's (manufactured by Cell Marque) H. Pylori on their instruments. The protocol is specific to the instruments but involves a routine HIER step and Avidin/Biotin blocking. The antibody itself is ready-to-use but is available from Cell Marque as a concentrate as well. T

RE: [Histonet] Helicobacter Pylori antibody

I have ordered mine from Cell Marque. We use it at 1/50 dilution with a 30 minute incubation at RT on the Dako Autostainers. Works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 __

RE: [IHCRG] Re: [Histonet] Helicobacter pylori IHC stain

:30 AM To: jtay...@meriter.com; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: [IHCRG] Re: [Histonet] Helicobacter pylori IHC stain We use Biocare's Rabbit Polyclonal (1:150) with the red chromagenreally cleaned up all the background..the docs love it! Ro

Re: [Histonet] Helicobacter pylori IHC stain

We are also trying Biocare's mouse monoclonal with red chromagen. Organisms look like HP on steroids! Beautiful... Konni Black - Original Message - From: To: ; ; Sent: Thursday, November 12, 2009 7:30 AM Subject: Re: [Histonet] Helicobacter pylori IHC stain We use Bioc

Re: [Histonet] Helicobacter pylori IHC stain

We use Biocare's Rabbit Polyclonal (1:150) with the red chromagenreally cleaned up all the background..the docs love it! Roxanne -Original Message- From: Taylor, Jean To: 'ih...@googlegroups.com' ; 'histonet@lists.utsouthwestern.edu' Sent: Tue, Nov 10, 2009 4:41 pm Subject:

RE: [Histonet] Helicobacter control slides

www.americanmastsertech.com , Item: CSHEL25, Helicobacter Pylori control slides. Josie Britton HT Cheshire Medical Center Keene, NH Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.ut

Re: [Histonet] Helicobacter

, and it is an understandable position. René J. --- On Thu, 9/18/08, Matt Roark <[EMAIL PROTECTED]> wrote: From: Matt Roark <[EMAIL PROTECTED]> Subject: Re: [Histonet] Helicobacter To: histonet@lists.utsouthwestern.edu Date: Thursday, September 18, 2008, 10:46 AM Thanks everyone

Re: [Histonet] Helicobacter

, Matt Roark <[EMAIL PROTECTED]> cc Subject Re: [Histonet] Helicobacter The reason why the pathologists are usually reluctant to use (+) cases, is because if they are used as a (+) controls they will be probably used up totally within a short period of time and IF there is some sort of l

Re: [Histonet] Helicobacter

a block to return to and make additional sections for litigation purposes, and it is an understandable position. René J. --- On Thu, 9/18/08, Matt Roark <[EMAIL PROTECTED]> wrote: From: Matt Roark <[EMAIL PROTECTED]> Subject: Re: [Histonet] Helicobacter To: histonet@lists.utsout

Re: [Histonet] Helicobacter

Thanks everyone! I also thought that we should be using positive patient controls but our pathologist wasn't to keen on the idea for some reason. But now with all of your responses maybe we will be. Thanks again! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center

RE: [Histonet] Helicobacter

We take a + case from our micro dept and the plate it off for us. We grow 4-6 plates and then swab the growth off and suspend it in broth. The broth is sectioned, processed and placed in block. This will last us for a couple years at a time. Mike -Original Message- From: [EMAIL PROTECTED]

Re: [Histonet] Helicobacter

We always used positive cases, selected by our pathologists. René J. --- On Wed, 9/17/08, Matt Roark <[EMAIL PROTECTED]> wrote: From: Matt Roark <[EMAIL PROTECTED]> Subject: [Histonet] Helicobacter To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 17, 2008, 1:39 PM What is everyon

RE: [Histonet] Helicobacter

We use positive cases from our GI biopsies. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 MarshallSlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Messa

Re: [Histonet] Helicobacter

Why not use a positive case from your laboratory? Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Matt Roark" <[EMAIL PROTECTED]> 09/17/08