Colleen,
The antigen expression you are getting in the human tonsil may not be
expressed in the mouse femur, plus u have to make sure the ab u are using
cross reacts with mouse tissue, if it is anti human it may or may not react
in mouse, the ab data sheet should indicate species reactivity. The
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
koelli...@comcast.net
Sent: Thursday, August 15, 2013 10:17 PM
To: Colleen Forster
Cc: Histonet
Subject: Re: [Histonet] IHC after EDTA decalcifying
Could I ask exactly
Hi Colleen,
We use ChelatorCal from BBC Biochemical, which contains EDTA. It is very
gentle and retains antigenicity for IHC stain. We really like it.
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
Could I ask exactly what you are looking for in mouse femur and human tonsil
the tonsil I assume is a control? For what? Whatever you are looking for is
obviously in human tonsil. Are you positive it is in murine femur. I used to
stain for 15-20 targets in EDTA decaled mouse femurs but
