Re: [Histonet] IHC and oven temperature

2015-05-02 Thread Joelle Weaver
ation. > > > > Regards, > > Tony > > > > ____________ > > From: Joelle Weaver [joellewea...@hotmail.com] > > Sent: Saturday, 25 April 2015 5:51 AM > > To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna > >

Re: [Histonet] IHC and oven temperature

2015-05-01 Thread Tony Henwood (SCHN)
Sent: Friday, 1 May 2015 7:51 AM To: Tony Henwood (SCHN); Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I have recently reviewed literature about sacrifice microwave fixation. Proteins in the living brain are denatured in as little as .3 seconds, stopping

Re: [Histonet] IHC and oven temperature

2015-05-01 Thread Tony Henwood (SCHN)
: Rene J Buesa [rjbu...@yahoo.com] Sent: Friday, 1 May 2015 5:56 AM To: Tony Henwood (SCHN); Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and oven temperature What about HIER at 95-98ÂșC? "Everybody" uses it to "enhance" epitopes detection. Is there not an int

Re: [Histonet] IHC and oven temperature

2015-04-30 Thread Rene J Buesa
O; Preiszner, Johanna Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for

RE: [Histonet] IHC and oven temperature

2015-04-24 Thread Joelle Weaver
, HTL (ASCP) QIHC > From: tony.henw...@health.nsw.gov.au > To: wdesalvo@outlook.com; preis...@mail.etsu.edu > Date: Fri, 24 Apr 2015 09:43:59 +0000 > Subject: RE: [Histonet] IHC and oven temperature > CC: histonet@lists.utsouthwestern.edu > > Hi temp dry

RE: [Histonet] IHC and oven temperature

2015-04-24 Thread Tony Henwood (SCHN)
edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo@outlook.com] Sent: Tuesday, 21 April 2015 1:56 AM To: Preiszner, Johanna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and oven temperature Dry heat compared to wet heat. Do not "d

Re: [Histonet] IHC and oven temperature

2015-04-20 Thread WILLIAM DESALVO
Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes Sent from my iPhone > On Apr 20, 2015, at 8:48 AM, Preiszner, Joha