RE: [Histonet] IHC mystery

2013-08-09 Thread HERRINGTON, SHEILA
...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Friday, August 09, 2013 10:13 AM To: Cindy Pyse; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC mystery Hi Cindy, I don't use the Dako system or any of the antibodie

RE: [Histonet] IHC mystery

2013-08-09 Thread Truscott, Tom
Hi Cindy, I don't use the Dako system or any of the antibodies you mention, but this kind of sounds like an antibody diluent problem, where either what you dissolve the antibody in doesn't spread out evenly on the slide or the antibody doesn't dissolve well in the diluents you use. In an automat