Luxol fast blue staining is dependent on the polarity of the solvent. Weak
staining can occur if the solvent becomes too polar, i.e. if sections are taken
down to water rather than 95% alcohol prior to putting in the staining
solution. Also check the amount of water in the ethanol or methanol us
True, if you solution is old it may not give the blue color that you want.
Are you doing the overnite procedure? I have found this to be the best. If your
oven isn't holding at 60º C you could use a waterbath.
Andi G
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
The only thing I think now refers to the "age" of your solution. If you prepare
it yourself or if bought and near the "expiration date" probably that could be
another cause of the problem.
René J.
From: Tim Wheelock
To: "'histonet@lists.utsouthwestern.edu'"
S
