Re: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Rene J Buesa
Use a 65ºC MP paraffin from Merk René J. From: "Louro, Pedro" To: Rene J Buesa ; Lucie Guernsey ; histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 2:32 PM Subject: RE: [Histonet] Mixing Paraffin Brands Just out of curiosity, what paraffin would people out there

Re: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Lucie Guernsey
bra Siena [ > dsi...@statlab.com] > Sent: Wednesday, June 19, 2013 12:29 PM > To: Lucie Guernsey; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Mixing Paraffin Brands > > Hi Lucie, > > Paraplast Plus has DMSO and Paraplast Xtra doesn't. It may be best to > infil

RE: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Elizabeth Chlipala
June 19, 2013 12:29 PM To: Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mixing Paraffin Brands Hi Lucie, Paraplast Plus has DMSO and Paraplast Xtra doesn't. It may be best to infiltrate with the Plus which is softer and then embed with the Paraplast Xtra whic

RE: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Jack Ratliff
- National Society for Histotechnology > From: jgarfi...@lifecell.com > To: lou...@princeton.huntingdon.com; rjbu...@yahoo.com; lguern...@ucsd.edu; > histonet@lists.utsouthwestern.edu > Date: Wed, 19 Jun 2013 14:17:22 -0500 > Subject: RE: [Histonet] Mixing Paraffin Brands > CC:

RE: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Garfield, Jacqueline
@lists.utsouthwestern.edu Subject: RE: [Histonet] Mixing Paraffin Brands Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates? We are currently using parapalst plus and was thinking of changing to a "harder paraffin" Any thoughts??

RE: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread DiCarlo, Margaret
: Wednesday, June 19, 2013 14:33 To: Rene J Buesa; Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mixing Paraffin Brands Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates? We are currently using parapalst plus and

RE: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Manfre, Philip
Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louro, Pedro Sent: Wednesday, June 19, 2013 2:33 PM To: Rene J Buesa; Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mixing Paraffin Brands Just out

RE: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Manfre, Philip
Lucie, Apparently I only responded to you directly. I am re-sending my response in case anyone else on the net is interested. Phil. Lucie, Personally, I would not mix them, as they have somewhat different properties, such as the melting point. I think you are asking for trouble, or

RE: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Louro, Pedro
tonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 19, 2013 2:24 PM To: Lucie Guernsey; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mixing Paraffin Brands Each paraffin has some additives to improve either its p

RE: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Debra Siena
Hi Lucie, Paraplast Plus has DMSO and Paraplast Xtra doesn't. It may be best to infiltrate with the Plus which is softer and then embed with the Paraplast Xtra which is a bit harder. I don't usually recommend mixing them especially if you only have a few bags. Thanks Debbie Siena 800.442.35

Re: [Histonet] Mixing Paraffin Brands

2013-06-19 Thread Rene J Buesa
Each paraffin has some additives to improve either its penetration rate or density to section. Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different. Preparing the block with the mixture will probably cause so tr