I think that the propidium iodide will stain the nuclei or DNA, similar
to DAPI, so this mounting media will counterstain the nuclei and its
visible in the FITC wavelength (488), while if you conterstain with DAPI
it is excited with a different wavelength, therefore it might interfere
with the
Hi Laurie,
Propidium iodide is a DNA intercalator that fluoresces red (ex 488nm).
Regards,
Merced
--On Thursday, May 20, 2010 11:13 AM -0700 Laurie Colbert
laurie.colb...@huntingtonhospital.com wrote:
We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting
Actually if I could make a minor correction to your statement: propidium
iodide is excited by green light at 488nm but emits in the red portion of
the spectrum (620nm)...
--On Thursday, May 20, 2010 12:48 PM -0600 Liz Chlipala
l...@premierlab.com wrote:
I think that the propidium iodide
