Altho' retired for 2 years, we had a protocol that retained all parts of the
eye quite well. Fixation was in 3% glutaraldehyde (diluted in H2O), 4 deg.C
for overnight. Don't extend the fixation as this will cause the tissue to be
too hard. Wash for about 1hr in running tap water and process
@lists.utsouthwestern.edu
Subject: Re: [Histonet] Mouse eyes
Altho' retired for 2 years, we had a protocol that retained all parts of the
eye quite well. Fixation was in 3% glutaraldehyde (diluted in H2O), 4 deg.C
for overnight. Don't extend the fixation as this will cause the tissue to
be too hard. Wash for about
I used to cut entire eyes of fish and lizards embedded in paraffin with great
results. The only thing is that on those (many years ago) days I used clearing
with Canada balsam followed by a short wash in benzene (not very safe) before
the paraffin infiltration.
René J.
--- On Wed, 7/29/09,
Do they also want undetached retina? If so, do not use NBF to fix.
Paula Pierce, BA, AAS, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway Ave.
Moore, OK 73160
405-759-3953
Specializing in whole eye histology. All species.
From: Jo-Ann Bader,
