tonet@lists.utsouthwestern.edu
Onderwerp: Re: [Histonet] PMS2
Diane,
When I was doing PMS2 I was using BD's mouse antibody @ 1:10
Retreived with Dako's TRS in a steamer for 40 min,
incubated the antibody for 60 minutes
and I used a labelled polymer for the detection (Dako's Envision +
Mouse)
It w
I don't know whose antibody you are using, but Leica-Microsystems recently
released a PMS2 mAb.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford,
Diane,
When I was doing PMS2 I was using BD's mouse antibody @ 1:10
Retreived with Dako's TRS in a steamer for 40 min,
incubated the antibody for 60 minutes
and I used a labelled polymer for the detection (Dako's Envision +
Mouse)
It was difficult to work up.
Good Luck,
Dana Settembre, HT ASCP
Im
