onet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicholas
David Evans
Sent: Saturday, 1 August 2009 5:37 AM
To: 'DELIA GARCIA'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Please Help!
We fix skin by sandwiching it between two spong
I do several skin frozens every week and I know exactly how frustrating it can
be when they roll! However, the technique I use is simple :
As the edge of the skin section that is rolling is about to make contact
with the knife/blade, put your soft brush bristles on top of the section and
ke
We fix skin by sandwiching it between two sponges soaked in either 4% PFA
or 0.4%PFA (for paraffin or cryo respectively) and compressed in a
cassette. This flattens the skin. Following several hours fixation the
skin is usually flat and rigid enough to handle and embed. It can then
either by dehydr
You might have some luck trying this -
Wipe out the cryostat with a dryer sheet to remove the static. This trick works
for me alot. Sometimes you need to repeat it a few times during cutting. Wiping
the cold plate withthe dryer sheet works wonders for me!
Good Luck!!
Stacey
--- On Fri, 7/31/
I always find it interesting to discover the vast sea of temperaments out
there that is elucidated so clearly in the light of email responses. The
same email can provoke anger, inflict hurt, or elicit laughter from
different people. :-)
With that in mind studies have shown (so I'm told by a fr
It was pretty funny alright. One for the Histonet Posts of Shame. There
are a few of us that could be runners-up with Ford. haha
Mark
On Fri, Mar 27, 2009 at 7:13 PM, Emily Sours wrote:
> I thought the rant was pretty funny.
>
> Just repeat to yourself "it's an email list, I should really ju
I thought the rant was pretty funny.
Just repeat to yourself "it's an email list, I should really just relax."
Emily
--
prometheus, thief of light, giver of light, bound by the gods, must have
been a book.
-mark danielewski, house of leaves
___
Histon
I, for one, love how he begins all politely with "hopefully not too
offensive to those that may be sensitive to such replies" before getting
straight to "Are you crazy?" not even three paragraphs later. This is
classic trolling at its finest... congratulations Ford! I'll be sure to
keep your ra
...@lists.utsouthwestern.edu] On Behalf Of
fro...@bitstream.net
Sent: Thursday, March 26, 2009 7:51 PM
To: Jennifer Anderson
Cc: Histonet
Subject: Re: [Histonet] Please help! In dire need of user manuals
Dear Jennifer and any other Histonetters that are of like mind,
It's not quite "Flaming F
-Original Message-
From: Joe Nocito [mailto:jnoc...@satx.rr.com]
Sent: Friday, March 27, 2009 11:53 AM
To: fro...@bitstream.net; Jennifer Anderson
Cc: Histonet
Subject: Re: [Histonet] Please help! In dire need of user manuals
OUCH
JTT
- Original Message -
From:
To: "Je
27, 2009 11:53 AM
To: fro...@bitstream.net; Jennifer Anderson
Cc: Histonet
Subject: Re: [Histonet] Please help! In dire need of user manuals
OUCH
JTT
- Original Message -
From:
To: "Jennifer Anderson"
Cc: "Histonet"
Sent: Thursday, March 26, 2009 9:50 PM
Subject: Re:
: Histonet
Subject: Re: [Histonet] Please help! In dire need of user manuals
OUCH
JTT
- Original Message -
From:
To: "Jennifer Anderson"
Cc: "Histonet"
Sent: Thursday, March 26, 2009 9:50 PM
Subject: Re: [Histonet] Please help! In dire need of user manuals
Dear Je
The response below is completely and utterly disrespectful and downright rude
Steven Mello,HT(ASCP)
Anatomical Pathology Manager
> From: jnoc...@satx.rr.com
> To: fro...@bitstream.net; jander...@halozyme.com
> Date: Fri, 27 Mar 2009 10:52:53 -0500
> Subject: Re: [Histonet] Ple
OUCH
JTT
- Original Message -
From:
To: "Jennifer Anderson"
Cc: "Histonet"
Sent: Thursday, March 26, 2009 9:50 PM
Subject: Re: [Histonet] Please help! In dire need of user manuals
Dear Jennifer and any other Histonetters that are of like mind,
It's not q
Dear Jennifer and any other Histonetters that are of like mind,
It's not quite "Flaming Friday" yet, but it will be in just a few hours,
so I'll go ahead a little early with what I hope is a reply that may
enlighten, be consultative, and hopefully not too offensive to those that
may be sensitive t
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Thursday, March 26, 2009 9:59 AM
To: Daniel Schneider; Histonet
Subject: RE: [Histonet] Please help! In dire need of user manuals
I'm sure each of them will sell you a manual. So much for business
rela
: Re: [Histonet] Please help! In dire need of user manuals
since the vip 3000 was dinscontinued sometime late last century, id say
finding a manual from its vendor is gonna be hard...
http://www.sakura-americas.com/techsupport/VIP1000.html
Daniel Schneider wrote:
> Have you asked Sakura, Le
since the vip 3000 was dinscontinued sometime late last century, id say
finding a manual from its vendor is gonna be hard...
http://www.sakura-americas.com/techsupport/VIP1000.html
Daniel Schneider wrote:
Have you asked Sakura, Leica, and Microm?
It would be interesting if they wouldn't prov
tonet
Subject: Re: [Histonet] Please help! In dire need of user manuals
Have you asked Sakura, Leica, and Microm?
It would be interesting if they wouldn't provide manuals for their
equipment, used or not.
On Thu, Mar 26, 2009 at 11:18 AM, Jennifer Anderson
wrote:
> Hello Everyone.
>
Have you asked Sakura, Leica, and Microm?
It would be interesting if they wouldn't provide manuals for their
equipment, used or not.
On Thu, Mar 26, 2009 at 11:18 AM, Jennifer Anderson
wrote:
> Hello Everyone.
>
>
>
> I our lab we've purchased all used equipment. None of these instruments
> ca
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