I don't believe that anyone responded to your inquiry. I used to make up a
Karnovsky's fixative (for TEM) (paraformaldehyde/glutaraldehyde) using the
powder form of paraformaldehyde. It is highly toxic. No one that I know of
does this anymore (or shouldn't). Now I buy the 16% solution of
I failed to mention that I make up my Karnovsky's and freeze aliquots. I have
never had a problem.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong
Sent: Monday, June 06, 2011 8:39 AM
To:
Suh et al.
I remember that the dogma on paraformaldehyde was that it must be made
fresh. However, I also remember that someone has actually compared fresh
to old (a week or two) and found no difference. This was published in
a peer-reviewed journal, I just don't remember which one.
Geoff
