Having read all the other comments, here's my 2.5 pence worth! Have you
tried a background reducing antibody diluent? You can get these from
DAKO or Menarini Diagnostics:
http://www.dako.com/uk/ar38/p107410/prod_products.htm.
Or MP-905-25 / MP-905-100, depending on the volume you require, from
Men
: Sarah Goebel; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Stupid Rabbit primaries!
Do you know for sure that its the Ab and not endogenous biotin,
peroxidase or Phosphatase? You can block for all those.
Otherwise there are a number of commercial blocking agents on the
market...try
Do you know for sure that its the Ab and not endogenous biotin,
peroxidase or Phosphatase? You can block for all those.
Otherwise there are a number of commercial blocking agents on the
market...try Biocare for a start (800)799-9499 or better yet, the
company that makes your antibody.
Good luck.
