Hugh thanks for your reply and I'll type my answers in blue below after the
questions:
_
From: Hugh Luk [mailto:hlu...@msn.com]
Sent: Thursday, February 03, 2011 6:21 PM
To: plu...@biopath.org
Subject: RE: [Histonet] TTF-1/background staining
Paula,
Incomplete IHC stainin
Hi Paula,
You mentioned the staining in a liver specimen using the cell marque
antibody against TTF-1. The clone named 8G7G3/1 that cell marque sells is
known to stain liver cells and liver cancer. Your pathologist might be
interested in knowing that. Here is a reference you can give him:
http:
Hi Paula
I do not believe that RTU Abs really exist. No company can produce an antibody
that is suitable for use by all laboratories taking in to consideration how
tissue can be handled differently from lab to lab. I have used many RTU Abs
which I have had to dilute to get optimal staining w
Hi Paula,
I am probably reading between the lines here but on your TTF protocol do you
use a streptavidin biotin detection system and if so do you block for
endogenous biotin, liver will have endogenous biotin where lung may not have as
much? thanks
Debbie Siena HT(ASCP)QIHC
Technical Manager
