...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Collette,
Nicole M.
Sent: Friday, July 23, 2010 2:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] Water collecting at bottom of sections
I use FisherBrand SuperFrost Plus charged slides, the only
I routinely flick my wrist holding the slide with the section I've
just picked up. Usually, this is enough force to release any water at
the bottom of the section. If that doesn't work I melt a tiny hole at
the bottom of the section with a heated probe and flick again. I'm sure
the
I experienced it almost daily and thought this was normal!?
I just dipped the slides gently on a paper towel right after getting
them on the slide and shaking, for the case I could not just shake it
off. Indeed, as I remember, the Super Frost Plus slides had to be dipped
a bit harder to let
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Friday, July 23, 2010 2:42 PM
To: nap...@mail.siscom.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Water collecting at bottom
I use FisherBrand SuperFrost Plus charged slides, the only time I have this
problem is when my waterbath is too hot, or if for some reason I use warmed
slides to retrieve my sections from the bath (like if you lay the unused slides
on the edge of the waterbath, or if you use the warm droplet
Am I the only person who paraffin sections by: cutting the sections,
grabbing the ribbon with forceps or paintbrush, placing it on a dry slide,
adding water underneath the ribbon with a pastuer pipet, and then placing
the slide on a slide warmer. The water will heat up and the paraffin ribbon
