RE: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Elizabeth Chlipala
Andrew There are aqueous permanent mounting medias such as Advantage Permanent Mounting Media from Accurate Chemical NB300A (516) 333-2221 its been years since I used it but it does work on some applications. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box

RE: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Cynthia Pyse
Andrew You could use Clearium from Leica. Clearium can either be coverslipped from xylene or isopropyl alcohol. Drying time from isopropyl is longer then xylene. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com

Re: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Victoria Baker
I think that it is called Crystal Mount - but apply to section, allow to harden dip slide in clearing media and coverslip. I know that there must be others out there as well. Good Luck On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman andrewcoleman...@gmail.com wrote: Hi all, We are

Re: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread Rene J Buesa
After staining dry the sections in an oven at 60ºC for 10 minutes. When completely dried, coverslip as usual. Beware of the mounting medium solvent because it may contain xylene as well. Use one mounting medium without xylene. René J. --- On Fri, 5/11/12, Andrew Coleman

RE: [Histonet] Xylene Substitute for Counterstain Clearing

2012-05-11 Thread gayle callis
] Xylene Substitute for Counterstain Clearing After staining dry the sections in an oven at 60ºC for 10 minutes. When completely dried, coverslip as usual. Beware of the mounting medium solvent because it may contain xylene as well. Use one mounting medium without xylene. René J. --- On Fri, 5/11/12