For this difficult tissue, and for any other for that matter, add to your
regular round water bath at 45ºC, 2 mL of liquid dish washer soap. The soap
will reduce the surface tension of the water and will allow the sections to
extend completely, eliminating any folds.
René J.
__
tonet-boun...@lists.utsouthwestern.edu] on behalf of Sheila Haas
[micropathl...@yahoo.com]
Sent: Friday, August 24, 2012 9:00 AM
To: Jennifer Sipes; Histonet
Subject: Re: [Histonet] folds in tissue
Try floating your sections on room temperature (in a shallow dish) water then
carefully, on a
Try floating your sections on room temperature (in a shallow dish) water then
carefully, on a slide, transfer to the waterbath. Sections will spread better
when they hit the warm water.
Good luck!
Sheila Haas
Laboratory Manager
MicroPath Laboratories, Inc.
From: Jennifer Sipes
To: Histonet
S
Under separate cover I am sending you something I wrote about some Tricks of
the Trade about flotation of paraffin sections.
René J.
--- On Mon, 10/12/09, Clare Thornton wrote:
From: Clare Thornton
Subject: [Histonet] Folds in tissue
To: "'histonet@lists.utsouthwestern.edu'"
Date: Monday, Oc
