Re: [Histonet] necropsy on freezed animal samples

2011-01-21 Thread Adam .
I recently had an animal from a previously undescribed mouse model die suddenly during the weekend. Since I couldn't send it off to necropsy, I stashed it in the refrigerator until Monday morning. According to the person who read the histology "Post-mortem degeneration limited evaluation of most ti

Re: [Histonet] necropsy on freezed animal samples

2011-01-21 Thread Geoff McAuliffe
If the tissue is not fixed soon after death the microscopic morphology will be terrible, no matter whether the animal is refrigerated or frozen. The person suggesting refrigeration/freezing is (obviously) not familiar with histological technique. As someone else on the list suggested, better pla

RE: [Histonet] necropsy on freezed animal samples

2011-01-21 Thread Fischer, Dolores
Kirshna, I wouldn't consider this to be a good practice. I would expect that if the experimental study is important, thought and planning would have dictated that, at time of necropsy, enough resources would be available to properly cover the necropsy procedure. If an animal unexpectantly die