-boun...@lists.utsouthwestern.edu] On Behalf Of Rachel M
Gonzalez
Sent: Monday, April 20, 2015 3:05 PM
To: Joelle Weaver
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] prevent wrinkles when cutting
Hi
The long ribbons are for R&D and QA purposes. Often we test under mult
Hi
The long ribbons are for R&D and QA purposes. Often we test under multiple
conditions 1-4 with multiple antibodies to the same tissue or multiple
tissue to optimize reagents. It quickly adds up.
Rachel
On Mon, Apr 20, 2015 at 3:28 PM, Joelle Weaver
wrote:
> Why are you cutting such a long
.
Pam Marcum
- Original Message -
From: "Joelle Weaver"
To: "Julio Benavides" , "Histonet"
Sent: Monday, April 20, 2015 2:28:47 PM
Subject: RE: [Histonet] prevent wrinkles when cutting
Why are you cutting such a long ribbon? You usually only a
No ice forms in fixed, paraffin embedded tissue blocks at usual temperatures
and length of chilling time and temperatures.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> Date: Mon, 20 Apr 2015 19:06:17 +0200
> From: j.benavi...@eae.csic.es
> To: histonet@lists.utsouthwestern.edu
> Subjec
Why are you cutting such a long ribbon? You usually only a need a series of 3-4
sections even for ribbon cutting. Might be easier to control if you don't try
to move such a long ribbon to the waterbath. Drag the shorter ribbon towards
you on the waterbath. Make sure the water is not too cool. Fa
If you process properly, no over dehydration, there is no reason to soak.
Keeping block cool will help in cutting. Sharp, clean blade a must. Correct
water bath temp and time are the most critical factors, after cutting, to
produce a wrinkle free section.
Sent from my iPhone
> On Apr 20, 2015
Check you blade angle. Keep your blocks cold, change your blade often and just
be patient.
Tom Podawiltz HT (ASCP)
AP Section Head
LRGHealthcare
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rac
How thick are you cutting? Is the block cold? Are you using disposable
knives? Are you moving the knife when you start sectioning? How fast are you
attempting to cut?
What kind of tissue are you cutting? Do you know which paraffin is being used?
There are many reasons for wrinkles and thes
First make sure your block is cold. Second, some models of microtome's and
blades don't cut well without dulling the blade a bit.(newer models don't seem
to need it.) Lastly some processors don’t fix as well as others you may need
to face the blocks in and soak them. Again some places soak in
