I used this method in a previous job which gave good results on 5um sections of
FFPE mouse lung. The eosin counterstain allowed easier navigation around the
tissue and meant the tissue was dark enough for the slide scanner to see
automatically.
* Dewax and rehydrate to distilled water
Try the one on stainsfile
(http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm). It gives
dark blue mast cell granules and is not removed with ethanol dehydration.
Bryan Llewellyn
Edwards, Richard E. wrote:
Hi All, your thoughts on the best toluidine blue method for mast
