： 2009-05-17 01:19:09
收件人： ti...@foxmail.com; 'Geoff McAuliffe'
抄送： Histonet@lists.utsouthwestern.edu
主题： RE: Re: [Histonet] 70% alcohol fixation of brain
U would be better off freezing the brain unfixed than trying to fix in alcohol
and freeze it, alcohol fixation is not recom
@lists.utsouthwestern.edu
Subject: Re: Re: [Histonet] 70% alcohol fixation of brain
thanks to the reply.
some antigens will be damaged by PFA fixation and can not be retreieval.
the shock frozen section with acetone is of quite bad tissue quality. therefore
I am trying alcohol!
2009-05-16
TF
发件人
@lists.utsouthwestern.edu
主题： Re: [Histonet] 70% alcohol fixation of brain
Greetings TF:
Good luck freezing a brain that is full of alcohol! Have you checked the
freezing point of alcohol?
Why are you doing this?
Immersing a whole brain in 70% alcohol, why??
30% sucrose is a cryoprotectant so the
> Good luck freezing a brain that is full of alcohol! Have you checked the
freezing point of alcohol?
> Why are you doing this?
I think that moire often than not, people ask before they think ;-)
On Fri, May 15, 2009 at 11:56 AM, Geoff McAuliffe wrote:
> Greetings TF:
>
> Good luck freezing a
Greetings TF:
Good luck freezing a brain that is full of alcohol! Have you checked the
freezing point of alcohol?
Why are you doing this?
Immersing a whole brain in 70% alcohol, why??
30% sucrose is a cryoprotectant so the brain is not full of holes from
ice crystals.Alcohol defeats this purpo
