Re: [Histonet] destain for IHC

Hi If you are using the peroxidase method the hydrogen peroxide step will remove the eosin and some of the haematoxylin. No need to do a decolorisation step before staining. As Gudren said the only problem may be not having coated slides. Good luck. Sadly retired histologist Tony Sent from my

Re: [Histonet] destain for IHC

Oh no!!! We cut all slides on + BUT you can do this. It’s what we do when we have H&E on plain slides. Whether you have a dry unstain from another lab that sent u a slide, or your H&E that you just took the coverslip off… Place it in. Coplin jar with 1-3 DROPS of formalin, cover, place In 60f

Re: [Histonet] destain for IHC

Hi Nancy, You should have no problem doing so. If you want proof, just run a couple of your IHC control sections through the H&E stainer as you would for a patient specimen (so right through to coverslipping) and then start the process of removing coverslip, hydration, destain in acid alcohol and t

Re: [Histonet] destain for IHC

We do restaining for IHC also, but without destaining at all. Hematoxylin doesn't matter because nuclei are stained after IHC with hematoxylin anyway. And Eosin gets lost through all the washing in buffer. The problem is, that HE section are usually mounted on non-adhesive slides, and they float aw

Re: [Histonet] destain for IHC

edical Center > 361 Whitney Ave., Holyoke, MA 01040 > Telephone: 413-322-4786 Fax: 413-322-4790 > charles.ba...@baystatehealth.org > > -Original Message- > From: Cooper, Brian > Sent: Wednesday, February 8, 2023 12:46 PM > To: Nancy Schmitt ; Histonet > > Subje

Re: [Histonet] destain for IHC

: Wednesday, February 8, 2023 12:46 PM To: Nancy Schmitt ; Histonet Subject: Re: [Histonet] destain for IHC We used to do this all the time in my old reference lab. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hos

Re: [Histonet] destain for IHC

We used to do this all the time in my old reference lab. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu