Hi everyone,
I recently ran a 2-8% gradient polyacrylamide gel. The 1kb Plus DNA
ladder had multiple bands. Does anyone know what might have caused
this and how to prevent it? I suspect that I might be caused by the
freeze thaw cycle. Also, some of my bands of my PCR samples were
slanted
Hi Everyone,
I was just wondering if anyone would have some "Insider's" knowledge about
the fixative "Histochoice". It is supposed to be a swanky non-formalin based
fixative for all and sundry, and the ingredients are supposed to be
"undisclosed" :). I am guessing it is some kind of
methanol/aceto
We routinely do phosphotyrosine blots (human proteins) with
anti-phosphotyrosine antibody clone 4G10 (many companies sell this). This is
the best and most cited antibody for phosphotyrosine and the most specific too.
It is of course not specific to any particular protein.
Jay
-Origi
Hi Priscila,
Phosphotyrosine may be quite easily detected; for a start, the PY20
antibody is a good choice.
pS and pT are very tricky when you do not know the context (motif) or
the identity of the modified protein, as pS and pT are not well
recognized by antibodies against the phosphorylated amin
Hi Ketut,
if you can't find any special procedure for your receptor in the
literature, I'd try simple fractionation:
first, disrupt your cells in a buffer that does not contain
detergents. This will remove the cytosol. Remainder should be able for
recovery at 15.000g. Wash the pellet a few times
Hello all,
I have to isolate G-PCR (G-protein coupled receptor) from ovarian cancer cell
line. I am looking for a protocol (buffer,etc) that not use
extraction/isolation kit (I could mix the buffer by my self) and not change its
native structure. There is a problem that my ultracentrifuge only c
Dear All:
I've been looking recently into phosphorylation of certain membrane proteins
after a peptide antibody (specific for my protein of interest) fails to
recognize the protein without prior treatment of the membrane (blotted
proteins) with phosphatase.
Most of the phosphorylation assays are
