Hello everyone
I wanted to check my protein concentration based on its extinction
coefficient by using nanodrop rather than doing Bradford or Lowry methods.
I was checking the article in one article, The protein concentrations were
estimated using the extinction coefficient of 2.70 at 280 nm for a
Hi Sudheer,
The units for the extinction coefficient are 1/(M*cm), which match the second
value you mention. I would use that number. The calculation is (OD 280nm) /
(extinction coefficient). This will give you a concentration in moles. You can
multiply by 10^6 to get the concentration in micro
