Retroviral vector packaging compatibility

2013-04-15 Thread Ed Siefker
I have a lentiviral system working in my lab (pInducer), my PI wants to know if we could use a pBabe based vector as well. pBabe apparently has MMLV based LTR instead of HIV. My question is whether the packaging constructs I have for lentiviruses will work for MMLV? I'm guessing that HIV and MML

RNA isolation from Formaldehyde fixed samples

2013-04-15 Thread Pow Joshi
Dear All, I have some cells fixed in 4% formalin (neutral I believe). I was wondering if anyone has tried any RNA extraction on such cell/tissue samples, and if you have any wisdom including kits, tricks, solutions, problems that you'd share with me. I would appreciate it hugely. Thank you much,