Re: RNA isolation from Formaldehyde fixed samples

2013-04-17 Thread Dr Engelbert Buxbaum
In article , pow.jo...@gmail.com says... > > On 16 April 2013 13:13, Shahrzad Jalali wrote: > > > Isolation of RNA from formalin fixed paraffin tissue is doable. I have done > > it. Use High Pure RNA paraffin Kit from Roche. > > > > Would you be able to share some of the details and experi

Re: RNA isolation from Formaldehyde fixed samples

2013-04-17 Thread Nick Theodorakis
On Tuesday, April 16, 2013 12:48:56 PM UTC-4, Pow Joshi wrote: > On 16 April 2013 00:00, DK wrote: > > > > > In article , Pow Joshi < > > > pow.jo...@gmail.com> wrote: > > > >Dear All, > > > > > > > >I have some cells fixed in 4% formalin (neutral I believe). I was > > > wondering > > > >

Re: RNA isolation from Formaldehyde fixed samples

2013-04-17 Thread Pow Joshi
On 17 April 2013 14:03, Pow Joshi wrote: > > > On 17 April 2013 10:54, Nick Theodorakis wrote: > >> On Tuesday, April 16, 2013 12:48:56 PM UTC-4, Pow Joshi wrote: >> > On 16 April 2013 00:00, DK wrote: >> > >> > >> > >> > > In article , Pow >> Joshi < >> > >> > > pow.jo...@gmail.com> wrote: >> >

Re: RNA isolation from Formaldehyde fixed samples

2013-04-17 Thread Shahrzad Jalali
I had followed exactly the kit direction to isolate RNA from formalin fixed paraffin embedded tissue. I am not sure how it will work if you use formalin fixed tissue...you would probably need to give a try. On Wed, Apr 17, 2013 at 10:54 AM, Nick Theodorakis < nick.theodora...@gmail.com> wrote: >

Re: RNA isolation from Formaldehyde fixed samples

2013-04-17 Thread Pow Joshi
On 17 April 2013 10:54, Nick Theodorakis wrote: > On Tuesday, April 16, 2013 12:48:56 PM UTC-4, Pow Joshi wrote: > > On 16 April 2013 00:00, DK wrote: > > > > > > > > > In article , Pow > Joshi < > > > > > pow.jo...@gmail.com> wrote: > > > > > >Dear All, > > > > > > > > > > > >I have some cells