Dear Experts,
I am attempting to precipitate small amounts of (total) protein from a
fruit juice concentrate. It looks quite much like honey. My plan is to
dilute it 1x with water (to reduce viscosity) and then saturate it
with ammonium sulfate. As the solution contains high amounts of sugars
(mos
Hi Wo,
I was wondering if you could simply use the crude extract to spin down and
create a sucrose density gradient...that will allow you to pick out some
proteins within the gradient, and then the remaining may go over for
ammonium sulphate precipitation ? ...I've no idea how this would work, but
What do you want to use the protein for?
I had a very dilute, yet viscous extract of flowers that I wanted to use for
western blotting. Carbohydrates made it visous, interfered with protein assays,
and made the samples run quite poorly in a gel. I ended up using a phenol
extraction method follo
Hi Pow, long time no see :)
My big problem is that there is almost no protein in the sample -> I won't see
any bands. I expect about 1mg from 50 ml. I have started with 50ml of fruit
juice concentrate, diluted with 50ml water and then added ammonium sulfate
until saturation: No visible turbidit
ubject: Re: Ammonium sulfate precipitation from solutions with high sugar
content
Hi Pow, long time no see :)
My big problem is that there is almost no protein in the sample -> I won't see
any bands. I expect about 1mg from 50 ml. I have started with 50ml of fruit
juice concentrate,
Hi Michael, I do not need active protein, I need it for a western. I'll give it
a try, thanks!
Wo
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and here's one more, from sugarcane :)
http://onlinelibrary.wiley.com/doi/10.1002/elps.200900779/pdf
on quick view it seems to me that they follow the method that Michael
suggested, however, they also seem to have comparative yields from
different methods ... hope that helps
Pow
On 1 May 2012 11:5
well, here's a plant phloem/Xylem sap protein isolation I found
http://www.springerlink.com/content/h8622j4747246137/#section=82175&page=4&locus=23
They use Centricon ultra filtration step to remove the sugars it seems to
me followed by TCA/acetone precipitation
On 1 May 2012 07:18,
In article ,
novalidaddr...@nurfuerspam.de says...
>
> Hi Pow, long time no see :)
>
> My big problem is that there is almost no protein in the sample ->
Iwon't see any bands. I expect about 1mg from 50 ml. I have started with
50ml of fruit juice concentrate, diluted with 50ml water and then a
