In article ,
silverstein.jos...@gmail.com says...
>
> I wish that people would be more respectful and just help. We don't know
> each other and I'd like to think of the listserve as a symbiotic
> relationship where we can all learn without being told we are stupid.
The issue was not that the OP
Cathal Garvey wrote:
>Personally I've forgotten plenty of supposedly "foundational" stuff from
>primary school that simply holds no value to me, including what was then a
I think the main thing, scientist have to be able to do, is to gain
new knowledge they need for their projects. I learned a l
Silverstein
Sent: Thursday, March 24, 2011 3:55 PM
To: WS
Cc: meth...@magpie.bio.indiana.edu
Subject: Re: Can anyone please tell about slope function
I feel his/her pain in hearing "you should check this source and that, this
is simple"... I do agree this subject is a bit ridiculous to ask,
Personally I've forgotten plenty of supposedly "foundational" stuff from
primary school that simply holds no value to me, including what was then a
very firm grasp of the Irish language. I'd like to know better Irish than I
do, but without needing it the skill simply atrophied away. If someone were
On 25/03/2011 05:53, DK wrote:
All this sounds fine and generally correct but let's not forget the obvious:
It is 5th or 6th grade material we are talking here. *That* makes quite
a difference. Lotsa implications.
It really depends on the intent of the question. Are they asking because:
1)
I feel his/her pain in hearing "you should check this source and that, this
is simple"... I do agree this subject is a bit ridiculous to ask, but I have
posed questions to the listserve before about protocols that seem to be so
common that a real detailed SOP isn't available unless your lab has bee
Dear Jay,
I think you got me a bit wrong. This was not an attempt to subverse
the presentation of measured data by using "politically incorrect"
methods.
As those colorimetric assays usually are not supposed to be a thesis
of itself, one usually is interested in quick and accurate results
(actuall
h 21, 2011 10:43 PM
To: meth...@magpie.bio.indiana.edu
Subject: Re: Can anyone please tell about slope function
In article , sudheer babu
wrote:
>Hi all
>Can anyone please tell what is the exact function and use of SLOPE , which
>calculates by y=mx+c formula. I found that slope defines
On Mar 21, 4:42 pm, "Jayakumar, R"
wrote:
> H!! the protein concentrations are on the X-axis and OD on the Yaxis.
> Always dependent variables on the Y-axis.
> Jay
Dear Jay,
Yes, it's correct. The goal of the method is not to produce
politically correct graphics but to get a useful (i.e.)
]
Sent: Monday, March 21, 2011 8:19 AM
To: meth...@magpie.bio.indiana.edu
Subject: Re: Can anyone please tell about slope function
Dear Sudheer,
the slope is the "m" in your equation. In real life, colorimetric
protein measurement standard curves sometimes get a bit far from
linear, so i
SLope is a function of how much the dependent variable Y changes for every unit
increase in the value of the independent variable X. So the line may be
negative or positive (negative if Y and X are inversely correlated). Knowing
that, imagine that the slope of the line you are trying to calcul
Dear Sudheer,
the slope is the "m" in your equation. In real life, colorimetric
protein measurement standard curves sometimes get a bit far from
linear, so in times of computerized curve fits by eg excel,
sigma{plot, stat} or openoffice.org, you might be better off with a
2nd order fit (y=ax2 + bx
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