RE: Immunochemistry question (probably dumb)

2011-06-21 Thread Jayakumar, R
...@magpie.bio.indiana.edu Subject: Re: Immunochemistry question (probably dumb) On Mon, 20 Jun 2011, DK wrote: > > But no matter how messy all of this is and what kind of complexes did > eventually from at their particular dilutions, any claim of being able to > distinguish proteins A and B is bound

RE: Immunochemistry question (probably dumb)

2011-06-21 Thread Jayakumar, R
BE A TYPO error. Jay -Original Message- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of WS Sent: Monday, June 20, 2011 5:18 PM To: meth...@magpie.bio.indiana.edu Subject: Re: Immunochemistry question (probably dumb) Dear Peter

Re: Immunochemistry question (probably dumb)

2011-06-21 Thread Peter Ellis
On Mon, 20 Jun 2011, DK wrote: But no matter how messy all of this is and what kind of complexes did eventually from at their particular dilutions, any claim of being able to distinguish proteins A and B is bound to be total unadulterated bullshit. Because no matter what, there ALWAYS be some co

Re: Immunochemistry question (probably dumb)

2011-06-20 Thread WS
Dear Peter, this question is not dumb. If complete antibodies were used, one should have amplified the other (and vice versa). That's what people usually do to amplify signals! Apparent differences in the stained structures (I assume some sort of detection by photography, video or confocal scanni