...@magpie.bio.indiana.edu
Subject: Re: Immunochemistry question (probably dumb)
On Mon, 20 Jun 2011, DK wrote:
>
> But no matter how messy all of this is and what kind of complexes did
> eventually from at their particular dilutions, any claim of being able to
> distinguish proteins A and B is bound
BE A TYPO error.
Jay
-Original Message-
From: methods-boun...@oat.bio.indiana.edu
[mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of WS
Sent: Monday, June 20, 2011 5:18 PM
To: meth...@magpie.bio.indiana.edu
Subject: Re: Immunochemistry question (probably dumb)
Dear Peter
On Mon, 20 Jun 2011, DK wrote:
But no matter how messy all of this is and what kind of complexes did
eventually from at their particular dilutions, any claim of being able to
distinguish proteins A and B is bound to be total unadulterated bullshit.
Because no matter what, there ALWAYS be some co
Dear Peter,
this question is not dumb. If complete antibodies were used, one
should have amplified the other (and vice versa). That's what people
usually do to amplify signals!
Apparent differences in the stained structures (I assume some sort of
detection by photography, video or confocal scanni