Re: Sterilised LB media getting some medicine smell, while I was growing E.coli culture

2011-09-24 Thread sudheer sangeetham
Hi Prasad Thank you for the reply, may be your interpretation is right. In your case when strange smells comes, could you able to express the protein? Thank you in advance Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. ___

Re: Sterilised LB media getting some medicine smell, while I was growing E.coli culture

2011-09-23 Thread Prasad Senadheera
rom: methods-boun...@oat.bio.indiana.edu > [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of sudheer sangeetham > Sent: Thursday, September 22, 2011 12:12 PM > To: meth...@magpie.bio.indiana.edu > Subject: Sterilised LB media getting some medicine smell, while I was growing > E.co

RE: Sterilised LB media getting some medicine smell, while I was growing E.coli culture

2011-09-22 Thread Jayakumar, R
...@oat.bio.indiana.edu] On Behalf Of sudheer sangeetham Sent: Thursday, September 22, 2011 12:12 PM To: meth...@magpie.bio.indiana.edu Subject: Sterilised LB media getting some medicine smell, while I was growing E.coli culture Hi Guys I am curious to know one thing that, I sterilised the LB media

Sterilised LB media getting some medicine smell, while I was growing E.coli culture

2011-09-22 Thread sudheer sangeetham
Hi Guys I am curious to know one thing that, I sterilised the LB media (which was little more diluted). I inoculated the cultures with suitable antibiotics and while I was measuring the OD of bacteria I could smell some medicine was there from the media, I really dont understand why it was like tha