Re: protein estimation

2013-04-11 Thread Dr Engelbert Buxbaum
In article , d...@no.email.thankstospam.net says... > > In article , Vladimir > Gainullin wrote: > >Is it not true that after a standard curve is created (using a more > >accurate method) one can use the A280 to estimate protein concentration > >given that the sample is more or less similar? >

Re: protein estimation

2013-04-09 Thread Vladimir Gainullin
Is it not true that after a standard curve is created (using a more accurate method) one can use the A280 to estimate protein concentration given that the sample is more or less similar? On Mon, Apr 8, 2013 at 8:34 PM, DK wrote: > In article , Sudheer > Sangeetham wrote: > >Hello all > > > >I

Re: protein estimation

2013-04-09 Thread kaj . stenberg
DK wrote: > In article , Sudheer > Sangeetham wrote: > >Hello all > > > >I would like to quantify the protein concentration. I checked the nanodrop > >manual in that they have given that I can estimate the protein by measuring > >directly at 280 nm by giving extinction coefficient value without

Re: protein estimation

2013-04-08 Thread Nick Theodorakis
On Monday, April 8, 2013 4:57:29 AM UTC-4, Sudheer Sangeetham wrote: > Hello all > > > > I would like to quantify the protein concentration. I checked the nanodrop > > manual in that they have given that I can estimate the protein by measuring > > directly at 280 nm by giving extinction coeffi

protein estimation

2013-04-08 Thread Sudheer Sangeetham
Hello all I would like to quantify the protein concentration. I checked the nanodrop manual in that they have given that I can estimate the protein by measuring directly at 280 nm by giving extinction coefficient value without doing bradford or lowry methods. I would like to choose measuring the p