Re: [Samtools-help] samtools sort output

2016-03-09 Thread Tommy Carstensen
Hi Carol, I believe the current version is 1.3. Version 0.1.19 is quite dated. Best wishes, Tommy From: carol white mailto:wht_...@yahoo.com>> Reply-To: carol white mailto:wht_...@yahoo.com>> Date: Wednesday, 9 March 2016 12:48:00 To: Samtools Help mailto:samtools-help@lists.sourceforge.net>> S

[Samtools-help] samtools sort output

2016-03-09 Thread carol white
Hi,Is it possible to output samtools sort in sam format? According to the documentation, yes but does it depend on the version (0.1.19)? I get -O not recognized. Cheers, Carol -- Transform Data into Opportunity. Accelera

Re: [Samtools-help] samtools PL

2016-03-09 Thread Tommy Carstensen
Hi Yuzhang, I think you miscalculated the permutations. Here VCF specs with the answer to your question: http://samtools.github.io/hts-specs/VCFv4.3.pdf Off the top of my head, answer is that PLs are (R is REF and A is ALT): RR,RA1,RA2,RA3,A1A1,A1A2,A1A3,A2A3,A2A2,A3A3 I could be wrong about th

[Samtools-help] samtools PL

2016-03-09 Thread yuzh...@ablife.cc
Hi dear: Thanks for your attention.I got some problems when using samtools. The VCF file contain an entry: Supercontig_1.1 104341 . G A,T,C 126 . DP=9;VDB=0.0030;AF1=1;AC1=2;DP4=0,0,4,4;MQ=60;FQ=-45 GT:PL:DP:GQ 1/1:159,18,0,158,8,155,158,8,153,155:8:33 There are 3 ALTs