[spctools-discuss] Mascot2XML with 2 or more databases

2013-03-14 Thread Mark Shifman
Hi: Is there a way to use the -D option with two or more fasta databases short of concatenating them together? We often do searches in Mascot with multiple databases ie 1::database.fasta 2::someotherdatabase.fast. Thanks in advance. Mark Shifman -- You received this message because you

[spctools-discuss] Re: Replicate data processing

2013-03-14 Thread Pavel
Hi Jason, Thank you for your answer. I just want to assure myself - is it possible to do this like selecting two or more files (pep.xml after a tandem search) in TPP with the selection of one interact.pep.xml output file? Since I use the Windows TPP this would be the best way if it is ok. I

[spctools-discuss] SINQ normalized spectral index tools for label-free quantitation

2013-03-14 Thread Greg Cary
Hi all, I've been reading a lot lately about a variety of label-free quantitation techniques. Unfortunately our data is poorly suited to MS1 peak extraction/integration (a la maxquant, skyline MS1 filtering etc.) and so we're very keen to implement some form of spectral counting (MS2 level)

[spctools-discuss] Number of unique peptides is more than the number of total peptides

2013-03-14 Thread Sandeep
Hi, I have recently started using peptide/proteinprophet. I noticed that in my data, there are several protein groups for which the number of unique peptidees is more than the number of total peptides (mostly the total number of peptides is 0). Is this data reliable? How to explain this.

[spctools-discuss] Peptide/ProteinProphet version for MS-GF+?

2013-03-14 Thread Jesse Meyer
Hi all, I am told that the most recent version of Peptide/ProteinProphet is compatible with MS-GF+ search output. Is this true? If so, where can I obtain it? I've already tried using TPP version 4.6.2 and xinteract fails upon calling PeptideProphet with the message that MS-GF+ is not