Re: [spctools-discuss] Extracting mzXML peaks

2013-04-01 Thread Dave Trudgian
Think I accidentally replied to author install of to the list... apologies if this ends up a double post. The script below extracts the following correct-looking m/z intensity values from the base64 string on stock perl v5.10.1 on ScientificLinux 6.3 64-bit. 300.282879284137 61862.0788574219 3

Re: [spctools-discuss] COMET searches with QExactive data

2013-04-01 Thread Dave Trudgian
Jimmy, Thanks for that info. Sounds great. I'll proceed based on the knowledge that'll be available. Cheers, DT On Monday, April 1, 2013 4:43:40 PM UTC-5, Jimmy Eng wrote: > > Dave, > > Mike Hoopmann implemented a nice fix for searches using small bins. The > sources current in Comet SourceF

Re: [spctools-discuss] Re: SINQ & normalized spectral index tools for label-free quantitation

2013-04-01 Thread Dave Trudgian
Greg, Sorry about that - I'll have it fixed tomorrow and contact you off list. The CPFP daemons are/were quite flaky, hence they break if any of various dependencies have any issue / the OS decides to upgrade and restart them etc. Am working on this, with help from others. Will get back to you

Re: [spctools-discuss] Re: SINQ & normalized spectral index tools for label-free quantitation

2013-04-01 Thread Greg Cary
Hi Dave, Thanks for following up. I had tried to use the CPFP demo server for a test with some of our data. Unfortunately I never made it past the search steps in the workflow. I just logged in and the two samples I'm trying to compare still report the status "SEARCHES QUEUED/RUNNING" and so

Re: [spctools-discuss] COMET searches with QExactive data

2013-04-01 Thread Jimmy Eng
Dave, Mike Hoopmann implemented a nice fix for searches using small bins. The sources current in Comet SourceForge trunk should be fully functional. One new parameter entry is titled "use_sparse_matrix" (see Comet version 2013.01 params in link below) which controls whether or not the sparse mat

Re: [spctools-discuss] COMET searches with QExactive data

2013-04-01 Thread Dave Trudgian
Hi Philip, Sorry to drag up an old thread, but I wondered if you had info about the effects on results of limiting Comet memory usage by using a large bin, vs using the 64-bit binary and small bin? We are primarily working with QExactive data here. I was thinking of adding Comet to our pipeli

Re: [spctools-discuss] Re: SINQ & normalized spectral index tools for label-free quantitation

2013-04-01 Thread Dave Trudgian
Hi Greg, Just a quick note that if you happen to have tried SINQ on CPFP and have had any problems with the demo server I apologize. I had been away for a while and not really monitoring it, so may not have worked for you. Am going to spend some time on CPFP soon. If you happen to try it on CP