Re: [spctools-discuss] LFQ

2020-10-02 Thread 'Luis Mendoza' via spctools-discuss
The other alternative is to run XPRESS in Label-free mode. For peaks where it can extract intensities, it will report two results: one for the integrated area with corresponding scan range, and one for the maximum peak intensity along with the scan number of that peak. You can view and export

Re: [spctools-discuss] Converting Orbitrap Eclipse files to mzML

2020-10-02 Thread Laura Muehlbauer
Both worked! Thanks so much! On Thursday, October 1, 2020 at 10:55:24 AM UTC-5 jwi...@gmail.com wrote: > It might also be worth trying the --ignoreUnknownInstrumentError flag in > your mzML command. > We had a similar problem with a new Thermo instrument where it wasn't > supported yet, and

[spctools-discuss] LFQ

2020-10-02 Thread vipulb...@gmail.com
I am a using TPP v5.2.0 Flammagenitus to analyze label free data generated on a Thermo QExactive machine. I want to perform LFQ, is there anyway to get Intensity values from the TPP. I am not sure about using StPeter since it is based on spectral counting. How can I perform LFQ in TPP ? --

Re: [spctools-discuss] X!Tandem- Help please

2020-10-02 Thread vipulb...@gmail.com
Hello Jimmy, Thanks for replying. Firstly, I am not sure about semi-tryptic search so I will set it to "no". Secondly, whenever I chose "tandem.params" file it automatically picks up the "isb_default_input_kscore.xml" rather than "isb_default_input_native.xml" as the default parameter. I do