[spctools-discuss] Why not use ASAPRatio to analyse quantitative data of 18O in TPP 4.31

2009-09-14 Thread Bright
command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" - lc -r0.5" exited with non-zero exit code: 1 QUIT - the job is incomplete command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 - Of -A-lc-r0.5 c:/Inetpub/wwwroot/ISB/data/HWJ/F002370.pep.xml&qu

[spctools-discuss] How to normalise peptide in TPP

2009-09-10 Thread Bright
Hi everyone I have two questions when using in TPP, The one is: Whether normalise peptide is necessary in analysis quantification mass spectrum data. The second is: How to use normalise in TPP. If you know please tell me. Thanks Bright

[spctools-discuss] How to change the workspace of TPP

2009-09-03 Thread Bright
Hi every I am not enough space to store raw data in C: disk , so I have to change work space in TPP but i can't. If you know please tell me . Thank Bright --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google G

[spctools-discuss] Can't get the last version TPP

2009-08-11 Thread Bright
Hi I want to download the last version TPP, but I can't open download web page: http://sourceforge.net/projects/sashimi/files/Trans-Proteomic%20Pipeline%20(TPP). Could you tell me the way to get the last version TPP, if you know. Thank very much B

[spctools-discuss] What the mean of "LIBRA (0)","LIBRA (1)".."LIBRA (n)" in .prot.xml

2009-07-16 Thread Bright
ld you tell how the understand the mean of LIBRA (n), -9.00 ± -9.00 and ± 99.99 if you know. Thank you Bright --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to th

[spctools-discuss] Why not run Combine Analyses in TPP v4.2 JETSTREAM rev 1, Build 200904071723 (MinGW)

2009-07-16 Thread Bright
SB/data/ShiDa/1.pep.xml c:/Inetpub/wwwroot/ISB/data/ShiDa/10.pep.xml interact.iproph.pep.xml" failed: Unknown error Thank you Bright --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss"

[spctools-discuss] Why not run Analyze Proteins when sample label by iTRAQ

2009-07-13 Thread Bright
bin\xinteract.exe -Ninteract.pep.xml -p0.05 - l7 -Ofp -Lcondition.xml-1 c:/Inetpub/wwwroot/ISB/data/LiuXiaoHui/ F003217.pep.xml" failed: Unknown error Command FAILED Can you tell me the reasons why happend it. I will appreciate for your help. Thank your Bright --~--~-~--~~---

[spctools-discuss] How to use the residue mass parameter in XPress and ASAPRatio

2009-06-08 Thread Bright
label model. The parameter one is "Change XPRESS residue mass difference" in XPRESS the other is "Specified residue mass" in ASAPRatio. If possible, could you show a example for me. Thank very much. Bright --~--~-~--~~~---~--~~ You received this mes

[spctools-discuss] What the meaning of "ASAPRatio: 1.94 ± 1.96(3)[1.35 ± 1.37]"

2009-05-30 Thread Bright
er the ratio of ASAPRatio is the differentia ratio of same protein in different sample? Thanks Bright --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send

[spctools-discuss] A question about using ASAPRatio

2009-03-23 Thread Bright
ead to failure of program but I can't sure. If you know please help me . Thank. Bright --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send ema

[spctools-discuss] A question about using ASAPRatio

2009-03-23 Thread Bright
ead to failure of program but I can't sure. If you know please help me . Thank. Bright --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send ema

[spctools-discuss] A question about using ASAPRatio

2009-03-23 Thread Bright
ead to failure of program but I can't sure. If you know please help me . Thank. Bright --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send ema

[spctools-discuss] Are these selectitems OK?

2009-03-16 Thread Bright
t;Run peptidePropet", selected "Run ProteinProphet afterwards" In "ASAPRatio Options": selected "Run ASAPRatio",selected "c" in list of "Change laeled residues" Finally clicked Run XInteract. Question: 1. Are these selec

[spctools-discuss] How to set selectitemes in analyze peptides web pape of TPP v4.2 for isotopic labeling

2009-03-05 Thread Bright
Hi everyone I need your help to deal with the question which how to set that selectitemes which in analyze peptides web pape of TPP v4.2. My sample was labeled by O18. Bright --~--~-~--~~~---~--~~ You received this message because you are subscribed to the

[spctools-discuss] How to analyse quantitation data of isotopic labeling

2009-03-02 Thread Bright
Hi everyone: I get some quantizations data of O18 labeling and convert them to *.pep.XML files but I don't know how to set correct property in Analyze peptides web page for analyze peptide data in *.pep.XML files. Please help me. Thanks --~--~-~--~~~---~--~~ You

[spctools-discuss] How to analyse quantitative data of O16 and O18

2009-02-19 Thread Bright
I get some search result of O16 and O18 using Mascoat search engine which files are *.dat. I want to get protein quantitative data which have been isotope labeling. If somebody knows how to deal with it please guide me to finish this work. Thank your Bright

[spctools-discuss] How to analyse quantitative data of O16 and O18

2009-02-19 Thread Bright
I get some search result of O16 and O18 using Mascoat search engine which files are *.dat. I want to get protein quantitative data which have been isotope labeling. If somebody knows how to deal with it please guide me to finish this work. Thank your Bright

[spctools-discuss] How to analyse O16 and o18 pepXML file in TPP

2009-02-18 Thread Bright
I get some pepXML files of O16 and O18 but I don't know how to use TPP tools ot analyse that files. The files come from Mascot search result. Thanks --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss

[spctools-discuss] Re: How to convert mzXML file to mgf file

2009-02-17 Thread Bright
I have solved it. The prgram mzXML2Search have been installed follow by TPP installed. Thank your very much for your kind. Bright On 2月17日, 上午2时13分, Matthew Chambers wrote: > It depends what you want to use the MGF for. I think the TPP pipeline > for the near future should continue

[spctools-discuss] Re: How to convert mzXML file to mgf file

2009-02-17 Thread Bright
I have solved it. The prgram mzXML2Search have been installed follow by TPP installed. Thank your very much for your kind. Bright On 2月17日, 下午10时04分, Matt Chambers wrote: > Do you mean mzXML2Search instead of Mascot2XML? I agree that spectrum > averaging should be a more universal f

[spctools-discuss] Re: How to convert mzXML file to mgf file

2009-02-17 Thread Bright
I have solved it. The prgram mzXML2Search have been installed follow by TPP installed. Thank your very much for your kind. Bright On 2月17日, 下午7时33分, Bill Nelson wrote: > We were never able to switch one of our labs over to TPP because they > use ABI Q-TOF with Mascot and the Mascot

[spctools-discuss] Re: How to convert mzXML file to mgf file

2009-02-17 Thread Bright
I have solved it. The prgram mzXML2Search have been installed follow by TPP installed. Thank your very much for your kind. Bright On 2月17日, 上午1时52分, "Brian Pratt" wrote: > The msconvert tool should handle that: > > To convert foo.mzxml to foo.mgf > >

[spctools-discuss] where to download mzXML2Search?

2009-02-17 Thread Bright
Could someone direct me to where to download where to download mzXML2Search? I can't seem to find one . Thanks -Bright --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group.

[spctools-discuss] How to convert mzXML file to mgf file

2009-02-16 Thread Bright
Hi everyone I can't deal with the problem that convert mzXML file to mgf file. Could your give me some ideal to over it. Thank. --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post t