[spctools-discuss] Re: SpectraST: option to disable adding AAs randomly to shuffled sequence for generating DECOY spectra?

2018-07-20 Thread Chih-Chiang Tsou
ys). > > Henry > > On Friday, July 20, 2018 at 5:10:29 AM UTC+8, Chih-Chiang Tsou wrote: >> >> Hi TPP developers >> >> I used SpectraST to generate decoy spectra and I noticed there are decoy >> spectra generated based on shuffled sequences with AAs add

[spctools-discuss] SpectraST: option to disable adding AAs randomly to shuffled sequence for generating DECOY spectra?

2018-07-19 Thread Chih-Chiang Tsou
Hi TPP developers I used SpectraST to generate decoy spectra and I noticed there are decoy spectra generated based on shuffled sequences with AAs added randomly. Is there option to disable it? Below is the message I got from spectrast: DECOY: Shuffle DFGLTSLALV to DSTLLGAFLVMGV. (3 AAs added

Re: [spctools-discuss] iProphet for Leucine/isoleucine IDs

2018-06-12 Thread Chih-Chiang Tsou
> On Tue, Jun 12, 2018 at 9:06 AM, Chih-Chiang Tsou < > chihchiang.t...@gmail.com> wrote: > >> Dear TPP developers, >> >> I have a question regarding how iProphet handles ambiguous I/L IDs from >> different search engine. I am using X!Tandem, Comet, and MSGF+ and

[spctools-discuss] iProphet for Leucine/isoleucine IDs

2018-06-12 Thread Chih-Chiang Tsou
Dear TPP developers, I have a question regarding how iProphet handles ambiguous I/L IDs from different search engine. I am using X!Tandem, Comet, and MSGF+ and combine the results using iProphet, and noticed that the three search engines have different rules ranking I/L hits. So how does

Re: [spctools-discuss] Can PeptideProphet take beyond top hit for probability calculation?

2017-04-04 Thread Chih-Chiang Tsou
al possibilities. First of all, which > version of the TPP are you on and what options are you using for > xinteract? It is possible that the repeating spectrum names are confusing > PeptideProphet. > > Thanks, > -David > > On Tue, Apr 4, 2017 at 11:39 AM, Chih-Chiang Tsou <

[spctools-discuss] Can PeptideProphet take beyond top hit for probability calculation?

2017-04-04 Thread Chih-Chiang Tsou
Hi Is there any option in xinteract to tell PeptideProphet take topN hits for each spectrum for probability calculation? Thanks, Chih-Chiang -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop

Re: [spctools-discuss] RTCalc (ANN)

2014-12-09 Thread Chih-Chiang Tsou
from the unmodified peptides. That said, learning algorithms such as this perform best when trained with enough data. If modified peptides are only a small part of the set they may not be very well characterized by the algorithm. -David On Sat, Dec 6, 2014 at 12:33 PM, Chih-Chiang Tsou

Re: [spctools-discuss] RTCalc (ANN)

2014-12-06 Thread Chih-Chiang Tsou
on peptide amino acid positional information and other peptide properties. They are different learning algorithms. -David On Fri, Dec 5, 2014 at 9:36 AM, Chih-Chiang Tsou chihchi...@gmail.com javascript: wrote: Hi David, I am trying to use rtcalc to train a prediction model directly

Re: [spctools-discuss] RTCalc (ANN)

2014-12-05 Thread Chih-Chiang Tsou
Hi David, I am trying to use rtcalc to train a prediction model directly from PepXML. Is the option PEPXML for that? it didn't work for me. Another question is what's the difference between coefficient file and ANN model file? Thanks, Chih-Chiang On Tuesday, April 23, 2013 12:29:18 PM UTC-4,