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--
l, but ProteinProphet wasn’t expecting it). Replace those tabs with
> spaces and it should all work.
>
>
>
> Brian
>
>
> --
>
> *From:* spctools-discuss@googlegroups.com [mailto:
> spctools-disc...@googlegroups.com] *On Behalf Of *QuanZe He
> *Sen
Dear Brian Pratt
I have zip up the pepXML and fasta files to Analyze_Proteins_Error.rar
and send it to ftp://insilicos.serveftp.net/pub. Thank you very much. I
will waiting your replay.
Best wish
Bright
2009/7/15 Brian Pratt
>
> Hard to say what is happening there - you might zip up the pepX
Thank very much
2009/6/10 Jimmy Eng
>
> iTRAQ is quantified based on ms/ms fragment peaks; use the Libra tool
> for that labeling method. ASAPRatio and XPRESS are not meant to work on
> iTRAQ data.
>
> As for O18 labeling, under the presumption that two O18 atoms are
> incorporated into the hea
Dear David Shteynberg
The modified mass of c-term is 21.0027 whether which construe by +1 Da
(-H) + 18 Da (O18) + 1 Da (H) + 1 Da (proton) or 17 Da (-OH) + 2 * (18 (O18)
- 16 (O16)). What is right?
Thanks.
Bright.
2009/3/26 QuanZe He
> Dear David Shteynberg
> Thank you for you
gt; 1
>
> Where m is mass and z is charge. Remember mass-specs measure
> intensities at different the mass-to-charge ratios.
>
>
> 2009/3/25 QuanZe He :
> > Dear David Shteynberg
> > I do it according to your advice to try specifying a mass of 21.0027
> for
>
David Shteynberg
>
> Hello,
>
> The unmodified mass of c-term is 17.0027. Looking up on the web I
> found that O18 label adds 4 daltons to the c-terminus. Please try
> specifying a mass of 21.0027 for ASAPRatio.
>
> Thanks,
> -David
>
> On Tue, Mar 24, 2009
Hi Brian Pratt
Thanks you for your attention. Now I find that the program will
successed if i change "Change Labeled residues" to "c" and doesn't set
"Specified residue mass 1:" to "c",4.1. I will get a normal outFile. But I
can't sure the result is righ. My sample is a mixture of label by O1
Dear David
Thank you for your response. I was downloaded "Proteomics informatics
Course Syllabus" from SPC, but it not detail informaction about how to
anaysis isotopic labeling. I will appreciate if you provide a templatefor
to me for anaysis isotopic labeling data by O18.
Best wish
Bright
