Re: [spctools-discuss] Error when running XPRESS

2012-12-13 Thread Brian Hampton
Hi Carlos, Do your searches using Tandem as Jimmy mentioned setting the light mod as a *static* on [ and K, then the difference between the light and heavy as the *variable* mod on [ and K. This is in the Tandem API here: http://www.thegpm.org/tandem/api/rmm.html. The Tandem API describes a stat

Re: [spctools-discuss] Error when running XPRESS

2012-12-12 Thread Jimmy Eng
Carlos, Unless I don't understand how XPRESS currently runs, I don't believe it will work on your separate light and heavy searches. This doesn't explain the failure to run error but it's worth mentioning up front. XPRESS expects a variable modification to denote the difference between your "lig

[spctools-discuss] Error when running XPRESS

2012-12-12 Thread Carlos
Dear all I am trying to analyze my dimethylation data using XPRESS tool in TPP but with no success so far... I did two XTandem searches for my mzXML files: one for light dimethyl (+28@N-terminus and @K) and another one for heavy (+34@N-terminus and @K) and merged them using the 'Analyze peptid