Re: [spctools-discuss] How does X!tandem handle the precursor charge in low resolution data

2010-04-03 Thread yinyin li
Thanks for the thorough explanation. I agree that this logic works quite successfully. At least in my hands, the data from LTQ without charge information work as well as these from orbitrap, in terms of protein ID. best yinyin On Fri, Apr 2, 2010 at 12:01 PM, Jimmy Eng wrote: > When a precursor

Re: [spctools-discuss] How does X!tandem handle the precursor charge in low resolution data

2010-04-02 Thread Jimmy Eng
When a precursor charge is not known, the logic that is used to analyze the data is as follows. If there are no peaks above the precursor m/z then the spectrum is assumed to be 1+ and analyzed as such. If there are peaks above the precursor m/z then the precursor ion is assumed to be multiply cha

[spctools-discuss] How does X!tandem handle the precursor charge in low resolution data

2010-04-02 Thread yinyin li
Hello: I collected some data from LTQ XP and converted them to mzXML by ReAdw. I noticed there is no precursor charge information, unlike the data from LTQ Orbitrap. Nevertheless, it seems X!tandem can still get it right. So i become very curious how it handles the charge. Does anyone know? Th