[spctools-discuss] Re: How to use the residue mass parameter in XPress and ASAPRatio

2009-06-11 Thread QuanZe He
Thank very much 2009/6/10 Jimmy Eng > > iTRAQ is quantified based on ms/ms fragment peaks; use the Libra tool > for that labeling method. ASAPRatio and XPRESS are not meant to work on > iTRAQ data. > > As for O18 labeling, under the presumption that two O18 atoms are > incorporated into the hea

[spctools-discuss] Re: How to use the residue mass parameter in XPress and ASAPRatio

2009-06-09 Thread Jimmy Eng
iTRAQ is quantified based on ms/ms fragment peaks; use the Libra tool for that labeling method. ASAPRatio and XPRESS are not meant to work on iTRAQ data. As for O18 labeling, under the presumption that two O18 atoms are incorporated into the heavy labeled peptides, you want to specify a 4 Da