Re: [spctools-discuss] protein prophet failing on Eclipse searches

2022-11-30 Thread 'David Shteynberg' via spctools-discuss
Hello, Generally this message means that you have no PSMs with good likelihoods of being correct in this analysis set. There are a few "usual suspect" reasons for why you would not observe any PSMs with non-zero probabilities among your results. 1. Poor quality data (e.g. empty sample) 2.

Re: [spctools-discuss] protein prophet failing on Eclipse searches

2022-11-29 Thread 'Luis Mendoza' via spctools-discuss
Hi, Congrats on the new instrument, and happy holidays as well! The error seems to indicate that there is no input data for ProteinProphet to work with; can you verify that the input file contains validated search results with probabilities above 0.2 ? Are there any other errors or warnings in

[spctools-discuss] protein prophet failing on Eclipse searches

2022-11-29 Thread cabarnes...@gmail.com
Hi all, Happy holidays! I have a new Eclipse in the lab and have been running my Comet + TPP pipeline successfully on a lot of data downloaded from PRIDE lately, but have run into an error with the new DDA data generated on the Eclipse. It seems to be failing at the protein prophet step and