To List Encapsulators,

 

Have any of you tried using a blender instead of an ultrasonic cleaner?  If
so, what were the results?  Perhaps Brooks could remind us of what the USC
does that a blender can't do as well.

 

Thanks,

Steve Y.

 

  _____  

From: Brooks Bradley [mailto:brooks76...@lycos.com] 
Sent: Thursday, August 27, 2009 9:08 AM
To: Silver-list@eskimo.com
Subject: CS>IMPORTANT COMMENT; Liposomal Encapsulation

 

Several list members have experienced difficulty in obtaining reliable,
accurate indications of the actual encapsulation percentages......using the
Do It Yourself technique which I outlined in an earlier post. NOTE: The
methodology outlined IS NOT RELIABLE as a true indication of achieved
encapsulation levels of vitamin C. The ONLY truly reliable means of,
accurately, knowing the degree of encapsulation is via Electron Microscopic 
observation. This entails preparation protocols/equipment beyond means of
most persons----even most laboratories. 
In my enthusiasm to avail list members of a "SIMPLE" protocol, which had
yielded results reliable enough to validate useful parameters....I outlined
a test which is 
NOT TRULY INDICATIVE of the encapsulated Vitamin C content. 
For those of you who followed my instructions for making a general
determination of the approximate, percentage levels of encapsulation, the
discrepancy in results....you may have achieved, ARE NOT YOUR FAULT and did
not result from a "broken protocol" on your part. 
Partial Explanation: There are just too many variables involved
(volumes/strengths/ratios) of the reactive materials, especially bicarbonate
and ascorbic acid fractions presenting. Just varying the velocity of mixing
can result in "marked" differences of the gas-release reaction. The gas
generation is NOT a true reflection of the percentage of non-encapsulated
Vitamin C fraction. 
While my intentions were of the highest order, my information was incomplete
and poorly constructed. For this, I deeply apologize. 
Although some may be able to replicate my results, the protocol proper, is 
too unreliable for general application. 
However, all is not lost. There is a VISIBLE, indicator of the general 
success of the experimenter's procedure. We HAVE determined, via electron
microscope examination by an associated laboratory....that almost any
solution achieved using the simple procedures outlined in my original post
for producing encapsulation.....yields 
>50% encapsulation. One excellent indicator is the degree of "apparent
homogenization". 
That is, the uniform, milky, appearance......and its "long-term" (days)
retention. 
I have no defensible excuse for my error....therefore, must appeal 
to the graces of the list members----for understanding. 
Sincerely, Brooks Bradley. 
P.S. I would be remiss if I failed to encourage ALL OF YOU to continue your
researches 
addressing use of this most promising protocol for encapsulation. The
majority of our 
investigations have yielded VERY powerful positive results. -- The Silver
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