Thanks very much for posting this.
 Do you know has any work been done on the
storage of DMSO in glass versus plastic. With DMSO's properties is it
safe to store it in plastic? perhaps if it is PET type hard plastic it
is OK. Would glass not be the safer option for DMSO?
With thanks in advance,
Sheila
Tuesday, September 20, 2005, 10:17:42 PM, you wrote:
TC> Please notice this: Very interesting!

TC> http://www.asapsolution.com/testresults.html

TC> Silver in Glass vs. Plastic Containers
TC> January 1, 2004 

TC> Non-Toxicity Test 

TC> In order to insure not only the best product, but also
TC> a safe product, American Biotech Labs hired an
TC> independent laboratory to do a toxicology study on the
TC> ASAP Solution. The test, called an LD-50 test, was
TC> performed in accordance with the guidelines of the
TC> Federal Hazardous Substances Act (FHSA) Regulations,
TC> 16 CFR 1500. 

TC> In the test work, the ASAP Solution was given to a
TC> number of both male and female test rats. The amount
TC> of ASAP Solution given to the rats was 5g/kg, or the
TC> equivalent of a 200 pound man taking 192 teaspoons of
TC> about 4 full 8 ounce bottles of the ASAP 10ppm
TC> solution at one time (the normal adult dosage is one
TC> or two teaspoons/day). 

TC> As a result of the test work, the independent
TC> laboratory made the following conclusion, "Under the
TC> conditions of this study, there was no mortality or
TC> significant evidence of toxicity observed in the rats.
TC> The test article (ASAP Solution) would not be
TC> considered toxic at a dose of 5g/kg by oral route in
TC> the rat." 

TC> American Biotech Labs Safety Data 

TC> American Biotech Labs has had five independent safety
TC> and toxicity tests completed on its 10 and 22 PPM
TC> silver products. The American Biotech Labs product was
TC> tested in animals at as much as 200 times the normal
TC> adult dosage, or the equivalent of an adult consuming
TC> 32 full ounces of the 10 ppm product at one sitting. 
TC> In conclusion to the animal tests, the independent
TC> medical testing laboratory stated that the ASAP
TC> Solution® was found to be completely non-toxic to the
TC> test animals.  The product was also tested for
TC> cytotoxicity in both human epithelial cells and also
TC> African green monkey or Vero cells, at both the
TC> regular 10 ppm level and also at the extra-strength 22
TC> ppm level.  In all four cytotoxicity tests the
TC> Amreican Biotech Labs' products were found completely
TC> non-toxic to both the human and Vero cells.   

TC> Silver in Glass vs. Plastic Containers 

TC> Introduction
TC> A great deal of controversy has arisen in the market
TC> place on the question of whether it is better to store
TC> silver solutions in glass versus plastic containers.
TC> There is a misconception that has prevailed in the
TC> market place that glass is better.  The idea that
TC> glass containers are better for storing products which
TC> contain silver has never been proven scientifically.
TC> In fact, it has been reported in other studies that
TC> glass may have a detrimental effect on silver
TC> products.   

TC> Test Work
TC> I have been conducting biological studies for 3 years,
TC> in the laboratory of a major private institution, on
TC> the use of silver products to kill and inhibit the
TC> growth of bacteria.  I have conducted thousands of
TC> tests on numerous strains of pathogenic bacteria. In
TC> the testing I have completed, I have used both glass
TC> (5 ml glass test tubes) and plastic (Falcon 5 ml
TC> polypropylene plastic test tubes). In some of the test
TC> work we found that there was a difference in the
TC> amount of silver that was needed to kill the bacteria
TC> when glass was used versus plastic test tubes.  In
TC> order to make sure this was the case, it was decided
TC> that the MIC tests (Minimum Inhibitory Concentration)
TC> should be replicated by more than one person and a
TC> direct comparison was made.  The MIC tests were
TC> performed in triplicate in both 5 ml 13X100mm glass
TC> test tubes and 5 ml Falcon polypropylene plastic test
TC> tubes.  Results of the bacterial (MIC) tests showed
TC> that Staphylococcus aureus was inhibited at 2.5 ppm
TC> when the MIC test was performed in the plastic test
TC> tubes.  S. aureus was inhibited at 5 ppm when the MIC
TC> test was performed in glass test tubes.  This
TC> suggested that the material with which the test tubes
TC> were made, specifically glass or plastic, may have
TC> effected the results of the MIC test.  Numerous other
TC> tests were also completed using larger concentrations
TC> of bacterium and in those tests it was found that
TC> there was no significant difference at all between
TC> using the glass test tubes versus plastic test tubes. 

TC> Other Studies
TC> The studies which I performed are not the only tests
TC> showing that glass, in some circumstances, may have a
TC> detrimental effect on silver products.  It has been
TC> noted in another independent study which has been
TC> cited by other researchers that silver can adsorb to
TC> glass (Chambers 1960; Thurman 1989). With this in
TC> mind, it may have been possible that the silver could
TC> have adsorbed to the surface of the glass test tubes
TC> reducing the concentration of available silver
TC> interacting with the bacteria which resulted in having
TC> to use a higher amount of silver to kill the bacteria
TC> when the glass test tubes were used.  

TC> Conclusion
TC> In the test work I have completed as well as in other
TC> available studies, it was found that glass may, in
TC> some cases, have a detrimental effect on silver
TC> products.  While it can be said that glass did not
TC> always show the detrimental effect, it can also be
TC> stated that we found no problems at all with using the
TC> plastic instead of glass.  Our tests, in conclusion
TC> with the other available independent study (1Chambers
TC> et al. and 2Thurman et al.), would suggest, by
TC> inference, that silver products should not be stored
TC> in glass containers which could reduce the available
TC> concentration of silver, but rather in a high quality
TC> plastic container. 

TC> Respectively, 

TC> David A. Revelli, MS  
 
TC> References 

TC> Chambers, C. a. C. P. (1960). The Bacteriological and
TC> Chemical Behavior of Silver in Low Concentration.
TC> Cincinnati, OH, Division of Water Supply and Pollution
TC> Control, U.S. Department of Health, Education, and
TC> Welfare. 
  
TC> Thurman, R. a. C. G. (1989). "The Molecular Mechanisms
TC> of Copper and Silver Ion Disinfection of Bacteria and
TC> Viruses." CRC Critical Reviews in Environmental
TC> Control 18(4): 295-314. 




        

        
                
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