Think of the TPP web GUI as a means of creating commands that you can run from the command line, where browser timeouts don't matter. Just copy the information from the "Attempting to run the following commands" window in the browser to a commandline window, it should run to completion there.
On Wed, Mar 10, 2010 at 2:27 PM, Eileen Yue <y...@ohsu.edu> wrote: > Hi Brian: > > Thank you for your advice! > In fact, after I load 5 or more fractions, the computer is so slow and I > wait so long (~1hours) and then, I just got the “Internet Explorer cannot > display the webpage” on the web and the TPP looks like shutting down > automatically. When I click more information on the web and it shows the > following information. Do you think it is due to the big size of the file > that I load or other reasons? Thank you! Eileen What you can try: > > > > Diagnose Connection Problems > > > > [image: More information] > > More information > > This problem can be caused by a variety of issues, including: > > - Internet connectivity has been lost. > - The website is temporarily unavailable. > - The Domain Name Server (DNS) is not reachable. > - The Domain Name Server (DNS) does not have a listing for the > website's domain. > - There might be a typing error in the address. > - If this is an HTTPS (secure) address, click Tools, click Internet > Options, click Advanced, and check to be sure the SSL and TLS protocols are > enabled under the security section. > > *For offline users* > > You can still view subscribed feeds and some recently viewed webpages. > To view subscribed feeds > > 1. Click the Favorites Center button , click Feeds, and then click the > feed you want to view. > > To view recently visited webpages (might not work on all pages) > > 1. Click Tools , and then click Work Offline. > 2. Click the Favorites Center button , click History, and then click > the page you want to view. > > > > > > > > *From:* spctools-discuss@googlegroups.com [mailto: > spctools-disc...@googlegroups.com] *On Behalf Of *Brian Pratt > *Sent:* 08 March 2010 16:16 > > *To:* spctools-discuss@googlegroups.com > *Subject:* Re: [spctools-discuss] how to analyze the big file size date > with TPP > > > > Hi Eileen, > > > > Perhaps you could start by posting the output from a failed TPP run (just > copy and paste from the TPP GUI). "Will not work" doesn't provide enough > information for anyone to make a helpful observation. > > > > Brian > > On Mon, Mar 8, 2010 at 3:59 PM, Eileen Yue <y...@ohsu.edu> wrote: > > Dear All: > Last time, I send this message to this mail list and want to get some > advice. I still do not solve the problem. Here I would like describe this > problem in detail and hope someone may give me some advice and help me solve > this problem. > > Basically, I use SCX-HPLC/MS to separate the SILAC sample, which is labeled > with isotope. I did off-line SCX-HPLC/MS and collected 30 fractions for the > LC/MS. The mass spectrometry is the new version from Thermo and called Velo. > After the sequest search with database, the results are very big file. All > the 30 fractions search files are almost 14G even after I zipped them. That > means each fraction is almost 1G. I tried to analyze one fraction with TPP > software and it works. When I load 5 or more fractions, TPP will not work > anymore. > > Does anyone have any advice how to analyze such big file size data with > TPP? Is there any other way to help me to analyze all the data. The major > things that I need combine all the fraction and get protein ASAP ratio. > > Thank you for your kind help > > Eileen > > -----Original Message----- > From: spctools-discuss@googlegroups.com [mailto: > spctools-disc...@googlegroups.com] On Behalf Of Eileen Yue > Sent: 04 March 2010 16:44 > To: spctools-discuss@googlegroups.com > Subject: [spctools-discuss] problem to analyze the VELO data with TPP > > Dear All: > I tried to use TPP to analyze the SILAC data produced from Velo under > Xcaliber 2.1. The sequest results for each fraction is very big and I have > total 30 fraction. I tried ONE fraction with TPP and it is fine to convert > the raw data into mzxml and sequest results to pepxml. When I try to analyze > more fraction together such as only 5 fraction, TPP does not work. Can > anyone tell me how to analyze my data and any advice for this situation? > > Thank you so much for your help > Eileen > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to spctools-disc...@googlegroups.com. > To unsubscribe from this group, send email to > spctools-discuss+unsubscr...@googlegroups.com<spctools-discuss%2bunsubscr...@googlegroups.com> > . > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to spctools-disc...@googlegroups.com. > To unsubscribe from this group, send email to > spctools-discuss+unsubscr...@googlegroups.com<spctools-discuss%2bunsubscr...@googlegroups.com> > . > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to spctools-disc...@googlegroups.com. > To unsubscribe from this group, send email to > spctools-discuss+unsubscr...@googlegroups.com<spctools-discuss%2bunsubscr...@googlegroups.com> > . > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to spctools-disc...@googlegroups.com. > To unsubscribe from this group, send email to > spctools-discuss+unsubscr...@googlegroups.com<spctools-discuss%2bunsubscr...@googlegroups.com> > . > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to spctools-disc...@googlegroups.com. To unsubscribe from this group, send email to spctools-discuss+unsubscr...@googlegroups.com. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
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