Hi, I am trying to convert the Thermo RAW files to a ProteinProphet XML file using TPP but in trouble...
1. I converted the RAW files into mzML files using msconvert included in the TPP 2. converted the mzML files into XML files using Comet (the Comet employed in TPP did not work, and I downloaded the latest Comet binary file and managed to convert them) 3. converted the resulting XML files into "interact.pep.xml" using "Analyze Peptides" in TPP, without any modifications in the parameters. 4. I finally tried to convert "interact.pep.xml" into "interact.prot.xml" using "Analyze Proteins" in TPP but failed. I have seen the following error message EXECUTING: c:\Inetpub\tpp-bin\ProteinProphet c:/Inetpub/wwwroot/ISB/data/interact.pep.xml c:/Inetpub/wwwroot/ISB/data/interact.prot.xml ProteinProphet (C++) by Insilicos LLC and LabKey Software, after the original Perl by A. Keller (TPP v4.7 POLAR VORTEX rev 1, Build 201405141849 (MinGW)) (xml input) (no FPKM) (report Protein Length) (using degen pep info) . . . reading in c:/Inetpub/wwwroot/ISB/data/interact.pep.xml. . . . . . read in 0 1+, 19860 2+, 5725 3+, 1328 4+, 147 5+, 0 6+, 0 7+ spectra with min prob 0.05 This application has requested the Runtime to terminate it in an unusual way. Please contact the application's support team for more information. Command FAILED RETURN CODE:768 My OS is Windows 8.1 (x64) Korean... and installed ActivePerl 5.16.3 (32 bit) Any recommendation for this? -Joungmin -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
