I tried the ProteoWizard but it did not work. The following error message 
appears in TPP. 

*EXECUTING: run_in c:/Inetpub/wwwroot/ISB/data; c:\Inetpub\tpp-bin\tandem 
c:/Inetpub/wwwroot/ISB/data/140728_JB_E1m.tandem.params *

X! TANDEM Jackhammer TPP (2013.06.15.1 - LabKey, Insilicos, ISB) 

Loading spectra (mzParser)............................... loaded.
Spectra matching criteria = 60476
Pluggable scoring enabled.
Starting threads .
This application has requested the Runtime to terminate it in an unusual way.
Please contact the application's support team for more information.

command "c:\Inetpub\tpp-bin\tandem 
c:/Inetpub/wwwroot/ISB/data/140728_JB_E1m.tandem.params" failed: No such process

*Command FAILED*
RETURN CODE:768

  Command 2 [Wed Sep 3 13:29:11 2014]       [ Show / Hide ]
*EXECUTING: run_in c:/Inetpub/wwwroot/ISB/data; 
c:\Inetpub\tpp-bin\Tandem2XML 
c:/Inetpub/wwwroot/ISB/data/140728_JB_E1m.tandem 
c:/Inetpub/wwwroot/ISB/data/140728_JB_E1m.tandem.pep.xml *

Failed to open input file 'c:/Inetpub/wwwroot/ISB/data/140728_JB_E1m.tandem'.

command "c:\Inetpub\tpp-bin\Tandem2XML 
c:/Inetpub/wwwroot/ISB/data/140728_JB_E1m.tandem 
c:/Inetpub/wwwroot/ISB/data/140728_JB_E1m.tandem.pep.xml" failed: Operation not 
permitted

*Command FAILED*
RETURN CODE:256

On Wednesday, September 3, 2014 10:24:19 AM UTC+2, Robert wrote:
>
>  Hi Jana,
>
> Did you try already the ProteoWizard (msconvert) for conversion? It has an 
> option for TPP compatibility.
>
> Best, Robert
>
> On 03.09.2014 02:29, Jana Horáková wrote:
>  
> Hi, 
> I would like to use TPP with Tandem to generate pepXML. I have encountered 
> a problem when trying to do the database search of my mzML file. I used the 
> AB Sciex MS Converter (release June 2012) and the batch command
>  
>  AB_SCIEX_MS_Converter WIFF "C:\AB SCIEX\ProteinPilot Data\Data\E1m.wiff" 
> -centroid MZML "C:\AB SCIEX\ProteinPilot Data\Data\E1m.xml" /index 
> /nocompression
> Pause
>  The conversion finishes without problems.
>
>  I tried leaving out /index /nocompression or generating -profile MZML 
> with the same outcome in TPP. The error message is pasted below.
>
>  X! TANDEM Jackhammer TPP (2013.06.15.1 - LabKey, Insilicos, ISB) 
>
> Loading spectra (mzParser).c:/Inetpub/wwwroot/ISB/data/140728_JB_E1m.mzml(1) 
> : error 2: Syntax error parsing XML.
>
> command "c:\Inetpub\tpp-bin\tandem 
> c:/Inetpub/wwwroot/ISB/data/140728_JB_E1m.tandem.params" failed: Unknown error
>
> *Command FAILED*
> RETURN CODE:63744
>  
>  Do you have any suggestions on how to solve the problem?
>
>  Thank you,
> Jana
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